Process for purifying bacterially produced M-CSF
US5466781A · kind A · utility
12Cited by
3References
23Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 8, 1993 |
| Grant date | Nov 14, 1995 |
| Priority date | — |
| Expiry date | Mar 8, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/53
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is described for producing M-CSF from bacteria. It includes: fermentation of bacteria containing M-CSF DNA; harvest of the fractions that contain the M-CSF protein (refractile bodies); primary recovery of the protein; solubilization and denaturation of refractile bodies; M-CSF refolding; purification by column chromatography and other methods; and formulation of the properly refolded M-CSF. This method is advantageous over prior methods in terms of yield and purity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.