Method of quantitative assay for 1,5-anhydroglucitol
US5468621A · kind A · utility
6Cited by
2References
7Claims
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Key dates
| Filing date | Feb 23, 1993 |
| Grant date | Nov 21, 1995 |
| Priority date | — |
| Expiry date | Feb 23, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/962
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A quantitative assay for 1,5-anhydroglucitol in a specimen is described. This assay comprises treating a specimen with a phosphorylating enzyme in the presence of 5 to 500 mM adenosine-5'-triphosphate in a pH range of 7.2 to 8.5, to selectively eliminate sugars other than 1,5-anhydroglucitol, and reacting the product of this reaction, without removing the residual ATP, with pyranose oxidase in the above pH range. This assay is quite rapid and suitable for performance using an automated device.
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