Method of isolating and purifying a biomacromolecule
US5468847A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 10, 1994 |
| Grant date | Nov 21, 1995 |
| Priority date | — |
| Expiry date | Mar 10, 2014 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01D2239/1291
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The invention provides a method of separating a biomacromolecule which comprises the steps of providing a separation system including a filter element which comprises a composite filtration medium, the composite filtration medium comprising a filtration layer on the upstream surface of which are located insoluble stationary phase particulates, the particulates being capable of binding to a biomacromolecule or class of biomacromolecules, a reservoir containing a solution mixture comprising at least one biomacromolecule as solute, and a pump and associated tubing to form a closed loop assembly, and recirculation pumping the solution mixture through the filter cartridge so as to bind the at least one biomacromolecule to the stationary phase particulate so as to form a biomacromolecule:stationary phase particulate product. An eluting solution can be pumped through the closed loop assembly which is capable of reversing the biomacromolecule:stationary phase particulate product binding interaction so as to liberate the biomacromolecule.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.