Particle-mediated transformation of mammalian unattached cells
US5470708A · kind A · utility
Inventors
Key dates
| Filing date | Apr 2, 1993 |
| Grant date | Nov 28, 1995 |
| Priority date | — |
| Expiry date | Apr 2, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/87
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of genetically transforming mammalian unattached cells is disclosed. The method begins by preparing copies of a nucleic acid construct and coating these copies onto biologically inert carrier particles. Mammalian unattached cells are isolated in a liquid suspension. The cell suspension is placed on a target surface, wherein the liquid is spread to a thin film on the target surface. In an alternative embodiment of the present invention, the liquid is spread onto a porous surface. The cells are bombarded with the construct-coated particles in such a fashion that some particles lodge in the interior of at least some of the cells. The existence and expression of the construct within the cell is verified.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.