Thermostable (1,3-1,4)-.beta.-glucanase

US5470725A · kind A · utility

Assignees

• Carlsberg A/S · DK
• Akademie der Wissenchaften der DDR · DE

Inventors

• Rainer Borriss · Glienicke, DE
• Jurgen Hofemeister · Quedlinburg, DE
• Karl Kristian Thomsen · Slagelse, DK
• Ole Olsen · Copenhagen, DK
• Dietrich Von Wettstein · Frederikssund, DK

Key dates

• Filing date: Aug 10, 1993
• Grant date: Nov 28, 1995
• Expiry date: Aug 10, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Novel hybrid thermostable (1,3-1,4)-.beta.-glucanases, their use in food manufacturing and feed manufacturing, DNA fragments encoding such glucanases, organisms expressing the DNA fragments and a method for producing the thermostable (1,3-1,4)-.beta.-glucanases. Hybrid fusion genes encoding Bacillus (1,3-1,4)-.beta.-glucanases were constructed, the gene products of which are more thermostable than any (1,3-1,4)-.beta.-glucanase known until now. The hybrid genes were constructed by reciprocal exchanges of the amino-terminal and carboxy-terminal parts of the .beta.-glucanase encoding genes from Bacillus amyloliquefaciens and Bacillus macerans. The resulting thermostable (1,3-1,4)-.beta.-glucanases retain a significant enzymatic activity at temperatures exceeding 65.degree. C. and at pH values below 5.0.