Chromosomal expression of non-bacterial genes in bacterial cells
US5470727A · kind A · utility
40Cited by
5References
1Claims
0Family size
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Key dates
| Filing date | Dec 21, 1993 |
| Grant date | Nov 28, 1995 |
| Priority date | — |
| Expiry date | Dec 21, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides compositions and methods for inserting a copy of a heterologous gene into the chromosome of a host cell such as E. coli through the use of a chromosomal transfer DNA, a circular, non-self-replicating DNA carrying a site-specific recombination site such as lambda att P. The host cell chromosome contains a second site-specific recombination site. When the chromosomal transfer DNA is introduced into the host cell, expression of an integration enzyme such as integrase causes the integration of the chromosomal transfer DNA into the host cell chromosome at the second recombination site.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.