Analysis of carbohydrates using 2-aminoacridone
US5472582A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jul 9, 1993 |
| Grant date | Dec 5, 1995 |
| Priority date | — |
| Expiry date | Jul 9, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44726
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides for the use of the fluorescent label 2-aminoacridone for use in separating carbohydrate mixtures and analyzing the structure of carbohydrates. Carbohydrates for analysis may be labeled by 2-aminoacridone and subsequently separated from one another by electrophoresis. The electrophoresis may be in one or two dimensions. Band produced by the electrophoresis may be visualized and quantitated directly by UV illumination or by means of a charge coupled device for photoelectric detection. 2-aminoacridone labelling of carbohydrate may also be used to analyze the structure of carbohydrates by cleaving (or adding to) various 2-aminoacridone labeled carbohydrates by carbohydrate modifying enzymes of known specificity, and subsequently separating the carbohydrates by electrophoresis in either one or two dimensions. The subject invention also provides for kits for performing 2-aminoacridone labelling and electrophoresis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.