Enzymatic method for synthesis of cellulose 1
US5472859A · kind A · utility
Inventors
Key dates
| Filing date | Aug 2, 1993 |
| Grant date | Dec 5, 1995 |
| Priority date | — |
| Expiry date | Aug 2, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01004
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses a method of synthesizing a novel form of cellulose I as well as methods of synthesizing a novel form of cellulose I in vitro. One method comprises contacting an activated saccharide substrate with an endoglucanase in an appropriate organic solvent/buffer ratio. The invention also encompasses a partially purified endoglucanase and a method of synthesizing cellooligosaccharides. A second method comprises contacting a nucleotide sugar with a purified glycosyl transferase in an appropriate buffer medium to insure polymerization and crystallization of parallel glucan chains from the enzyme/micelle complex to form cellulose I.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.