Method for preparing immobilized enzyme conjugates and immobilized enzyme conjugates prepared thereby
US5472861A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 9, 1995 |
| Grant date | Dec 5, 1995 |
| Priority date | — |
| Expiry date | Jan 9, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for preparing an immobilized enzyme conjugate, whereby the enzyme is treated with a polyfunctional amine reactive material for forming a treated enzyme-containing adduct before being immobilized on a solid support which has been contacted with a solution of a polyamine compound. The method is especially preferred for use with glucoamylase, fungal .alpha.-amylase and .beta.-amylase. Immobilized enzyme conjugates formed by use of this method include treated enzyme-containing adducts. The immobilized enzyme conjugates disclosed herein are more stable and the enzymes immobilized therein are more tightly-held than those otherwise obtained and provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.