Method of preparing solution enriched in EG III using low molecular weight alcohol, organic salt and inorganic salt
US5472864A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jan 13, 1995 |
| Grant date | Dec 5, 1995 |
| Priority date | — |
| Expiry date | Jan 13, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/945
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for preparing an aqueous solution enriched in EG III from an aqueous mixture containing cellulase proteins, xylanase and EG III is disclosed. The method involves adding an amount of a low molecular weight alcohol selected from the group consisting of ethanol, methanol, propanol and mixtures thereof to the aqueous mixture containing cellulase proteins, xylanase and EG III and an organic salt under conditions wherein substantially all of the cellulase proteins other than EG III and xylanase are precipitated out of the aqueous mixture. The method then involves removing the precipitate from the aqueous mixture so as to recover an aqueous supernate enriched in EG III. Next, the method involves adding an amount of an inorganic salt to the supernate produced in step b) so as to form a second precipitate and a second supernate and then finally collecting the second supernate from the second precipitate to obtain a supernate enriched in EG III.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.