Stable transformation of maize cells by electroporation
US5472869A · kind A · utility
109Cited by
2References
7Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 8, 1994 |
| Grant date | Dec 5, 1995 |
| Priority date | — |
| Expiry date | Jun 8, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8206
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method to increase the susceptibility of cultured Zea mays cells to stable transformation with recombinant DNA via electroporation, by pretreating the Zea mays cells with certain pectin-degrading enzymes, so that the cells retain their ability to regenerate fertile, transgenic Zea mays plants containing the DNA which is also heritable.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.