Biological support for cell cultures constituted by plasma proteins coagulated by thrombin, its use in the preparation of keratocyte cultures, their recovery and their transport for therapeutic purposes
US5474770A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 12, 1992 |
| Grant date | Dec 12, 1995 |
| Priority date | — |
| Expiry date | Mar 12, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S623/915
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
The present invention relates to a biological support for cell cultures formed by the coagulated mixture of a concentrate of plasma proteins and thrombin. The protein concentrate is obtained by precipitating fresh plasma with ethanol and contains balanced proportions of fibrinogen, Factor XIII and fibronectin. The thrombin concentration is adjusted to obtain the desired consistency of the support coagulated in the form of a film. The biological support is preferably used for preparing a culture of keratinocytes, recovering them in the form of a reconstituted tissue and transporting same. The reconstituted tissue is thus particularly suitable for use as a graft.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.