Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor
US5474895A · kind A · utility
175Cited by
17References
22Claims
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Key dates
| Filing date | May 13, 1993 |
| Grant date | Dec 12, 1995 |
| Priority date | — |
| Expiry date | May 13, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/703
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for the detection of nucleic acid using sandwich hybridization are provided. The target nucleic acids are captured by hybridization with oligonucleotides covalently attached to polystyrene solid supports to form complexes which are then hybridized to detection oligonucleotides to facilitate the analysis of the target nucleic acid sequences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.