Screening assay for the identification ov novel immunosuppressives using cultured T cells
US5474897A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 15, 1993 |
| Grant date | Dec 12, 1995 |
| Priority date | — |
| Expiry date | Nov 15, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6897
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for identifying compounds capable of inducing immunosuppression by inhibiting the CD28 signal transduction pathway and T cell comprises exposing cultured T cells to one or more test compounds. The T cells are obtained from a T cell line which stably incorporates DNA sequence comprising in reading frame an enhancer region responsive to a CD28-regulated nuclear binding protein and a marker gene. The cells are cultured under conditions which result in activation of both the T cell receptor and the CD28 receptor, resulting in enhanced expression of the marker gene. Test compounds which inhibit expression of the marker gene are considered as candidates for immunosuppressive drugs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.