Multilayer analytical element for salicylate assay
US5474907A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 25, 1994 |
| Grant date | Dec 12, 1995 |
| Priority date | — |
| Expiry date | Mar 25, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/805
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A multilayer analytical element for measuring salicylate is disclosed. The element has a support. The support is coated with the following layers, top down to the support: a) a spreading layer, b) a dye layer having a pH greater than 6.5 and comprising tyrosinase and a water-insoluble hydrazone coupling agent, c) a barrier layer that prevents passage of molecules having a molecular weight in excess of 5,000, and d) a reagent layer comprising salicylate hydroxylase and nicotinamide adenine dinucleotide (NADH), wherein the spreading layer comprises valproic acid, 8-anilino-1-naphthalenesutfonic acid (ANS) or the magnesium salt of 8-anilino-1-naphthalenesulfonic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.