Noncolorimetric histoculture method for predicting drug response of tumors
US5474909A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 7, 1992 |
| Grant date | Dec 12, 1995 |
| Priority date | — |
| Expiry date | Aug 7, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/52
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of evaluating the effectiveness of drugs in inhibiting the growth of tumor cells. A sample of a tumor is histocultured, a drug to be evaluated is added to the sample and the sample is incubated. A suitable tetrazolium salt is added and a frozen section of the sample is prepared. The section is stained with a fluorescent dye. The section is exposed to polarized light and the reflected light is measured. Then the section is exposed to fluorescent light and the light emitted by the dye is measured. These measurements are compared to a control and/or measurements from tests using other drugs and the relative effectiveness of the drug is evaluated, preferably by pixel analysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.