Immunometric determination of an antigen or hapten

US5476770A · kind A · utility

Assignee

• COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES · FR

Inventor

• Philippe Pradelles · Palaiseau, FR

Key dates

• Filing date: Jan 24, 1994
• Grant date: Dec 19, 1995
• Expiry date: Jan 24, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/822
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a process for the immunometric determination of an antigen or hapten. According to this process, contacting takes place (FIG. 1A) between the antigen or hapten (3) to be determined and the antibodies (2) fixed to a solid phase in order to immunologically bond the antigen or hapten with the antibody. This is followed (FIG. 1B) by immobilizing the antigen or hapten (3) by a covalent bond (4) to the solid phase (1) whilst releasing its epitope (FIG. 1C). This is followed by the contacting thereof (FIG. 1D) with labelled antibodies (5) and determination takes place (FIG. 1E) of the quantity of fixed labelled antibodies in order to deduce therefrom the initial hapten or antigen concentration. As a result of this stage of immobilizing and releasing the epitope, a high sensitivity is obtained using a single antibody.