Methods for the desalting of biological samples: a simple approach to eliminate disturbances in isoelectric focusing caused by the presence of salts
US5480526A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 7, 1994 |
| Grant date | Jan 2, 1996 |
| Priority date | — |
| Expiry date | Jun 7, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44795
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides new methods for desalting samples of ionic polymers or weak electrolytes, preferable ampholytes (e.g., peptides, proteins and glycoconjugates) or, compounds which can be transformed into these substances by complex formation. The methods of the present invention are based on the fact that salts can be removed from a sample by electrophoretically replacing the salts with ampholytes present in the ampholytic media of the anolyte and the catholyte or, with displacing cations and anions that have been introduced into the anolyte and catholyte, respectively, and which have mobilities less than the mobilities of the cations and anions to be removed from the sample. Although applicable to both small-volume and large-volume samples, the methods are particularly well suited for small-volume samples. Moreover, when applied to capillary electrophoresis, the methods described herein permit desalting of a sample in the same capillary tube as is used for the electrophoretic analysis. Alternatively, however, the sample can be withdrawn from the capillary tube following desalting and processed by techniques other than high-performance capillary electrophoresis (HPCE)…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.