Nucleic acid sequence amplification methods
US5480784A · kind A · utility
262Cited by
2References
6Claims
0Family size
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Key dates
| Filing date | Jul 10, 1990 |
| Grant date | Jan 2, 1996 |
| Priority date | — |
| Expiry date | Jul 10, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/158
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods of synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies. Nucleotide sequences of target nucleic acid portions and of primers are selected to minimize the ability of the primer to remain able to form a DNA primer extension product of for formation of an RNA:DNA primer hybrid and exposure to RNase H.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.