Water-soluble proteins modified by saccharides

US5480790A · kind A · utility

Assignees

• Boehringer Mannheim GmbH · DE
• Gesellschaft fur Biotechnologische Forschung mbH (GBF) · DE

Inventors

• Wilhelm Tischer · Peißenberg, DE
• Joachim Klein · Hochspeyer, DE
• Rolf Muller · Marburg, DE
• Stephan Engelke · Drensteinfurt, DE

Key dates

• Filing date: Apr 4, 1991
• Grant date: Jan 2, 1996
• Expiry date: Apr 4, 2011

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N11/082
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A water-soluble protein conjugate modified by saccharides is covalently bound to a carbohydrate backbone via a saccharide group. Such protein conjugates modified by saccharides can be produced in a simple way by polymerizing a vinylsaccharide and conjugating in a known way the poly(vinylsaccharide) obtained in this way with the protein, which is preferably an enzyme. The protein conjugates according to the present invention are stable over long time periods at a high enzymatic activity and also in aqueous solution and are thus particularly suitable for use in test kits.