Method for reproducing douglas-fir by somatic embryogenesis
US5482857A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 25, 1994 |
| Grant date | Jan 9, 1996 |
| Priority date | — |
| Expiry date | Feb 25, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N5/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention is a method for reproducing Douglas-fir by somatic embryogenesis using plant tissue culture techniques in a multistage culturing process. A suitable explant, typically the fertilized embryo excised from an immature seed, is first cultured on a medium that induces multiple early stage embryos. These are multiplied in a second culture having reduced growth hormones where the early stage embryos grow in size and vigor to advanced early stage embryos. The embryos may then be transferred directly to a cotyledonary embryo development culture containing an adsorbent such as activated charcoal. Preferably, they are first singulated by shaking in two or more liquid subcultures containing the adsorbent prior to placing them on the development medium. After several weeks somatic embryos having the appearance of zygotic embryos will have formed. These may be germinated before or after storage and transplanted to soil for further growth.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.