Method, composition and device for the determination of cholesterol using cholesterol oxidase obtained from bacterial strain NRRL B-18713
US5487978A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 5, 1991 |
| Grant date | Jan 30, 1996 |
| Priority date | — |
| Expiry date | Mar 5, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/805
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a kinetic or rate method for the determination of cholesterol in free or bound form with of a cholesterol oxidase enzyme obtained from Bacterial strain NRRL B-18713 which inherently has a high Michaelis and Menton, Km, of about 10-2 to 10-3 M for cholesterol and optionally, of cholesterol esterase. The method can measure oxygen consumption, hydrogen peroxide or cholestenone formed. The present invention also provides a test composition for the kinetic determination of cholesterol, comprising cholesterol oxidase with an inherently high Km of about 10-2 to 10-3 M for cholesterol and optionally cholesterol esterase and a system for the determination of hydrogen peroxide, cholestenone or oxygen consumption.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.