Method for increasing plasmid yield
US5487986A · kind A · utility
4Cited by
6References
3Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 10, 1994 |
| Grant date | Jan 30, 1996 |
| Priority date | — |
| Expiry date | Mar 10, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/69
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to an aqueous medium formulation which is capable of allowing the growth of a plasmid-producing host cell to a density greater than 30 at a reading of 600 nm and, in addition, enhancing plasmid production beyond that which would be expected at the cell densities achieved. The medium of the present invention comprises a carbon source in the concentration range of about 20 to about 40 grams per liter of the water component of the medium.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.