Process of producing fertile transgenic zea mays plants and progeny comprising a gene encoding phosphinothricin acetyl transferase

US5489520A · kind A · utility

Assignee

• Dekalb Genetics Corporation · US

Inventors

• Thomas R. Adams · Springdale Township, US
• Sheryl A. Chambers · Mystic, US
• Richard J. Daines · Ledyard, US
• William J. Gordon-Kamm · Urbandale, US
• Albert P. Kausch · Stonington, US
• Peggy G. Lemaux · Moraga, US
• Catherine J. Mackey · Old Lyme, US
• Mary L. Mangano · Westerly, US
• James V. O'Brien · Mystic, US
• Thomas Rice · Waterford, US
• T. Michael Spencer · Mystic, US
• William G. Start · Preston, US
• Nancy G. Willetts · Niantic, US

Key dates

• Filing date: Apr 26, 1994
• Grant date: Feb 6, 1996
• Expiry date: Apr 26, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S47/01
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.