Immobilization of biomolecules on a fluorocarbon surface with a poly(fluoroalkyl) sugar reagent
US5491083A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 5, 1994 |
| Grant date | Feb 13, 1996 |
| Priority date | — |
| Expiry date | Oct 5, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/816
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Poly(fluoroalkyl) sugar reagents are prepared containing a sugar such as a monosaccharide or a disaccharide to which are bonded a plurality of fluoroalkyl anchor groups capable of attaching to a fluorocarbon surface, and either a reactive group capable of covalent coupling to a biomolecule such as an enzyme or a charged group to form an ion-exchanger or a non-ionic group to give a neutral fluorosurfactant. A spacer may be between the reactive group and the sugar. The poly(fluoroalkyl) sugar reagents are strongly adsorbed onto fluorocarbon surfaces to provide supports for such applications as separation and immobilization of biomolecules such as enzymes, carrying out heterogeneous diagnostic assays, and preparation of biosensors.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.