Uses of green-fluorescent protein
US5491084A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Sep 10, 1993 |
| Grant date | Feb 13, 1996 |
| Priority date | — |
| Expiry date | Sep 10, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6897
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides a cell comprising a DNA molecule having a regulatory element from a gene, other than a gene encoding a green-fluorescent protein operatively linked to a DNA sequence encoding the green-fluorescent protein. This invention also provides a method for selecting cells expressing a protein of interest which comprises: a. introducing into the cells a DNAI molecule having DNA sequence encoding the protein of interest and DNAII molecule having DNA sequence encoding a green-fluorescent protein; b. culturing the introduced cells in conditions permitting expression of the green-fluorescent protein and the protein of interest; and c. selecting the cultured cells which express green-fluorescent protein, thereby selecting cells expressing the protein of interest. Finally, this invention provides various uses of a green-fluorescent protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.