Quantitative determination of lipid, and of co-existing two compounds
US5491093A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 9, 1992 |
| Grant date | Feb 13, 1996 |
| Priority date | — |
| Expiry date | Sep 9, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/105831
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Lipid is determined quantitatively by treating a sample with a surfactant in an aqueous medium to obtain a liquid sample dispersion, then reacting the dispersion with a reagent containing cupric ion and bicinchoninic acid, and measuring a colored state developed by complex formation of bicinchoninic acid with cuprous ion formed from cupric ion in the presence of lipid. This method can be effectively applied to determination of amphiphilic lipid having a tendency of gathering in an aqueous medium. Further, two different species interfering with each other in quantitative determination thereof are determined respectively by conducting two different determination methods on the same sample followed by calculation using the values obtained.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.