Coupled transcription and translation in eukaryotic cell-free extract

US5492817A · kind A · utility

Assignee

• Promega Corporation · US

Inventors

• David V. Thompson · Monona, US
• Thomas R. Van Oosbree · Madison, US
• Gregory S. Beckler · Portage, US
• John F. Van Herwynen · Stoughton, US

Key dates

• Filing date: Jun 27, 1994
• Grant date: Feb 20, 1996
• Expiry date: Jun 27, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for coupling transcription and translation from DNA using a solution comprising a eukaryotic cell-free extract comprising adding a sufficient amount of a magnesium compound to the extract to raise the magnesium concentration to a level where RNA is transcribed from DNA and RNA translates into protein.