Method of producing L-glutamic acid by fermentation
US5492818A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 5, 1994 |
| Grant date | Feb 20, 1996 |
| Priority date | — |
| Expiry date | Dec 5, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/843
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method of producing L-glutamic acid by fermentation, comprising the steps of culturing a mutant of an L-glutamic acid-producing microorganism of the genus Brevibacterium or Corynebacterium which has lower .alpha.-ketoglutaric acid dehydrogenase activity compared with the wild strains from which said mutant is derived, in a liquid nutrient culture medium containing biotin at a concentration of 10 to 1000 .mu.g/l without adding a biotin activity-suppressing substance thereto; producing and accumulating L-glutamic acid in the culture solution; and recovering L-glutamic acid from said culture solution. According to the method of the present invention, it is possible to industrially produce L-glutamic acid by fermentation in a more economical and efficient manner.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.