Apparatus and method for seeding endothelial cells
US5492826A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 10, 1993 |
| Grant date | Feb 20, 1996 |
| Priority date | — |
| Expiry date | Dec 10, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M23/08
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An apparatus and method for culturing living endothelial cells is provided. The apparatus comprises a cell growth container, which in a preferred embodiment is a synthetic graft, heat crimped at both ends. The apparatus further comprises a rotation container that may contain the cell growth container and is attached to a rotation apparatus. The apparatus further comprises fixturing apparatus, which in a preferred embodiment is a pair of o-ring members, that enhances a separated condition of the cell growth container within the rotation container. The method of the present invention comprises providing a culture that includes living cells and a growth medium, introducing the culture into a cell growth container, heat crimping the cell growth container to substantially seal the cell growth container, and positioning fixturing apparatus upon the external surface of the cell growth container. This assembly is inserted into a rotation container, which is rotated by a rotation apparatus.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.