Enzymatic resolution of .alpha.-tertiary carboxylic acid esters
US5492830A · kind A · utility
7Cited by
5References
12Claims
0Family size
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Inventors
Key dates
| Filing date | Jun 25, 1993 |
| Grant date | Feb 20, 1996 |
| Priority date | — |
| Expiry date | Jun 25, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P41/005
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the resolution of .alpha.-tertiary carboxylic acid esters by partial hydrolysis is disclosed. The partial hydrolysis is carried out by contacting the esters with an aqueous solution in the presence of a serine carboxypeptidase, and the hydrolysis product is separated from unreacted starting material to obtain the hydrolysis product or the unreacted starting material in enantiomerically enriched form. A novel ester hydrolase which is particularly useful in this method, and a nucleotide sequence encoding this enzyme, are also described.
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