Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5494810A · kind A · utility
884Cited by
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4Claims
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Key dates
| Filing date | Nov 22, 1994 |
| Grant date | Feb 27, 1996 |
| Priority date | — |
| Expiry date | Nov 22, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6858
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the cloning of the gene of a thermophilic DNA ligase, from Thermus aquaticus strain HB8, and the use of this ligase in a ligase chain reaction (LCR) assay for the detection of specific sequences of nucleotides in a variety of nucleic acid samples, and more particularly in those samples containing a DNA sequence characterized by a difference in the nucleic acid sequence from a standard sequence including single nucleic acid base pair changes, deletions, insertions or translocations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.