Enhanced indole biosynthesis
US5494816A · kind A · utility
6Cited by
0References
5Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jun 21, 1994 |
| Grant date | Feb 27, 1996 |
| Priority date | — |
| Expiry date | Jun 21, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P7/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
DNA molecules encoding a modified tryptophan synthase beta subunit are disclosed. When expressed in a recombinant host microorganism, these polypeptide analogs enable significant levels of intracellular indole production and accumulation. In the presence of an aromatic dioxygenase enzyme, the indole so produced can be converted to indoxyl, which upon exposure to air oxidizes to indigo.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.