Assay method for hemicellulases using a colored substrate
US5496707A · kind A · utility
0Cited by
5References
13Claims
0Family size
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Key dates
| Filing date | Apr 5, 1994 |
| Grant date | Mar 5, 1996 |
| Priority date | — |
| Expiry date | Apr 5, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/924
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides an assay method for hemicellulases comprising a) directly dyeing, using a reactive dye, an insoluble natural product, or a modified form of a natural fibre material; and b) adding the enzyme to the dyed product produced in step a) and, after a specific incubation period, separating the liquid component from the insoluble dyed product, e.g. by a simple filtration, and determining the amount of dyestuff liberated in the the separated solution by spectrophotometric means. A combined mixing and dispensing device for use in the method of the present invention is also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.