Methods of generating desired amino-terminal residues in proteins
US5496721A · kind A · utility
10Cited by
5References
7Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 9, 1994 |
| Grant date | Mar 5, 1996 |
| Priority date | — |
| Expiry date | May 9, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/95
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods of designing or modifying protein structure at the protein or genetic level to produce specified amino-termini in vivo or in vitro are described. The methods can be used to alter the metabolic stability and other properties of the protein or, alternatively, to artificially generate authentic amino-termini in proteins produced through artificial means. The methods are based upon the introduction of the use of artificial ubiquitin-protein fusions, and the discovery that the in vivo half-life of a protein is a function of the amino-terminal amino acid of the protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.