Methods for rapid microbial detection
US5498525A · kind A · utility
41Cited by
0References
7Claims
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Key dates
| Filing date | Apr 9, 1992 |
| Grant date | Mar 12, 1996 |
| Priority date | — |
| Expiry date | Apr 9, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/66
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of testing for target bacteria involves adding bacteriophage to a sample to infect the bacteria in the sample; killing extracellular bacteriophage without at the same time killing phage-infected bacteria; amplifying bacteriophage remaining in the sample; and causing the bacteriophage to infect reporter bacteria and thereby produce an observable signal. The reporter bacteria are genetically engineered to have an indicator gene which on expression gives rise to a detectable signal, wherein expression of the indicator gene is initiated on bacteriophage infection of the bacteria.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.