Bovine endothelial nitric oxide synthase nucleic acids
US5498539A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 2, 1992 |
| Grant date | Mar 12, 1996 |
| Priority date | — |
| Expiry date | Jul 2, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/0075
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The cloning, nucleotide and deduced amino acid sequences of the gene encoding endothelial nitric oxide synthase are described. The sequence contains binding domains for calcium/calmodulin, FMN, FAD and NADPH. The enzyme has a molecular weight of 133,413 M.sub.r. The amino terminal portion of the enzyme exhibits a proline-rich region and several sites for proline-directed phosphorylation as well as a potential substrate site for acyl transferase. DNA probes prepared from the nucleic acid sequence are useful in research and diagnostically to determine the level of nitric oxide synthase mRNA expressed by endothelial cells both in cell culture and in intact tissues. These probes are also useful for detecting genetic abnormalities. The nitric oxide synthase gene is transfected into blood vessels in vivo for enhanced synthesis of nitric oxide synthase, resulting in increased production of nitric oxide. The gene is also transfected into host cells that do not normally express the enzyme for production of endothelial nitric oxide synthase in large volumes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.