Bacillus lentus alkaline protease varints with enhanced stability
US5500364A · kind A · utility
59Cited by
4References
54Claims
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Key dates
| Filing date | Jun 2, 1994 |
| Grant date | Mar 19, 1996 |
| Priority date | — |
| Expiry date | Jun 2, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B20/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Mutant B. lentus DSM 5483 proteases are derived by the replacement of at least one amino acid residue of the mature form of the B. lentus DSM 5483 alkaline protease. The mutant proteases are expressed by genes which are mutated by site-specific mutagenesis. The amino acid sites selected for replacement are identified by means of a computer based method which compares the three dimensional structure of the wild-type protease and a reference protease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.