Particle bound binding component immunoassay
US5501949A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 15, 1994 |
| Grant date | Mar 26, 1996 |
| Priority date | — |
| Expiry date | Aug 15, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/824
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An immunoassay method for the detection or quantitation of an analyte suspected of being in a solution comprising: (a) combining said specimen, a first binding component, insoluble particles, and second binding component labelled with a signal generating material in a solid phase retention and separation apparatus having a sufficient pore size such that said particles are trapped within said filter yet permitting rapid passage of fluid therethrough in such a manner that an immunological reaction occurs if analyte is present in said specimen, resulting in the formation of an immunocomplex of insolublized first binding component:analyte:second labelled binding component on or within said filter means; (b) separating bound from unbound material; and (c) determining the presence and/or amount of signal produced which is correlative with the amount of analyte present in the solution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.