Process for producing fructose 2,6-bisphosphate and purification process thereof
US5501965A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 16, 1994 |
| Grant date | Mar 26, 1996 |
| Priority date | — |
| Expiry date | Mar 16, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a process for the production of fructose 2,6-bisphosphate which comprises effecting reactions among (i) fructose 6-phosphate, (ii) glucose, (iii) fructose or (iv) glucose 6-phosphate, ATP and a phosphate donor, in the presence of (i) fructose 6-phosphate 2-kinase (PFK 2) and an enzyme which converts ADP into ATP (ADP/ATP converting enzyme), (ii) PFK 2, an ADP/ATP converting enzyme, hexokinase or glucokinase and glucose 6-phosphate isomerase, (iii) PFK 2, an ADP/ATP converting enzyme and hexokinase or glucokinase, or (iv) PFK 2, an ADP/ATP converting enzyme and glucose 6-phosphate isomerase; to a process for the production of fructose 2,6-bisphosphate which comprises allowing diesterase to coexist in a solution containing fructose 1,2-cyclic, 6-bisphosphate; and to a process for the purification of fructose 2,6-bisphosphate which comprises adding zinc salt to a solution containing fructose 2,6-bisphosphate, removing formed precipitate of impurities and adding a zinc salt to the resulting solution to isolate formed precipitate of fructose 2,6-bisphosphate. According to the present invention, fructose 2,6-bisphosphate can be produced easily with a high yield …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.