Split ubiquitin protein sensor
US5503977A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 22, 1994 |
| Grant date | Apr 2, 1996 |
| Priority date | — |
| Expiry date | Apr 22, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed are novel compositions and methods useful for studying interactions between proteins. An N-terminal subdomain and a C-terminal subdomain of ubiquitin are linked to a pair of proteins or peptides to be examined for their ability to interact. When contacted with one another, a quasi-native ubiquitin moiety is reconstituted provided that the protein or peptide pair do, in fact, interact (bind) with one another. The quasi-native ubiquitin moiety is recognized and cleaved by ubiquitin-specific proteases after the last residue of ubiquitin. The cleavage at the quasi-native ubiquitin moiety within a linear protein fusion is the indication of interaction between the protein or peptide pair.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.