Method for detecting charged oligonucleotides in biological fluids
US5506103A · kind A · utility
3Cited by
4References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 16, 1993 |
| Grant date | Apr 9, 1996 |
| Priority date | — |
| Expiry date | Nov 16, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/101
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method for detecting and quantitating oligonucleotides with charged internucleotide linkages in biological fluids. In this method, a biological fluid sample is contacted with an anion exchange resin at from 40.degree. C. to 65.degree. C. for a time sufficient to enable oligonucleotides in the sample to adsorb to the resin. The absorbed oligonucleotides are then desorbed with a buffer having a salt concentration of about 1 M to 2.5 M and a pH in the range of about 6.5 to 7.5, the desorption being performed at about 40.degree.-65.degree. C. The oligonucleotides so released are then detected and quantitated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.