In situ assay of amplified intracellular mRNA targets
US5506105A · kind A · utility
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Key dates
| Filing date | Mar 22, 1994 |
| Grant date | Apr 9, 1996 |
| Priority date | — |
| Expiry date | Mar 22, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In an in situ hybridization assay, cells are fixed under conditions which preserve essential morphology, followed by transcription-based nucleic acid amplification of intracellular mRNA targets. The amplified targets are visualized by hybridizing labelled probes to the target sequences. The assay thereby provides a convenient method for detecting specific gene expression associated with morphologic characteristics of diagnostic significance.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.