Fusion peptides with binding activity for streptavidin

US5506121A · kind A · utility

Assignee

• Institut Fur Bioanalytik Gemeinnutzige Gesellschaft MBH · DE

Inventors

• Arne Skerra · Freising, DE
• Thomas Schmidt · Dransfeld, DE

Key dates

• Filing date: Nov 3, 1993
• Grant date: Apr 9, 1996
• Expiry date: Nov 3, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/22
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A peptide according to the invention comprises the amino acid sequence PA1 Trp--X--His--Pro--Gln--Phe--Y--Z, in which X represents any desired amino acid and Y and Z either both denote Gly, or Y denotes Glu and Z denotes Arg or Lys. A fusion protein according to the invention consists of the amino acid sequence of a complete protein, of a protein mutant such as a deletion mutant or substitution mutant, or of part of a protein linked to the sequence of a peptide of the invention. For the production of a recombinant protein by expression of a DNA sequence coding therefor in suitable host cells according to well-known methods, a DNA sequence is used which codes for a fusion protein and, if desired, the presence of the expression product is detected by means of a conjugate of streptavidin and a label or the desired protein is separated as a fusion protein by means of streptavidin affinity chromatography.