Purified thermostable Pyrococcus furiosus DNA ligase
US5506137A · kind A · utility
30Cited by
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6Claims
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Key dates
| Filing date | Jul 22, 1993 |
| Grant date | Apr 9, 1996 |
| Priority date | — |
| Expiry date | Jul 22, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/822
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Purified thermostable DNA ligase is described that catalyzes template-dependent ligation at temperatures of about 30.degree. C. to about 80.degree. C., and which substantially retains its catalytic ability when subjected to temperatures of from about 85.degree. C. to about 100.degree. C. The thermostable DNA ligase has an estimated molecular weight of 50,000 to 70,000 daltons. A preferred thermostable DNA ligase is described that was isolated from the archaebacteria Pyrococcus furiosus. Also described are plasmid vectors for producing recombinant thermostable DNA ligase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.