Recombinant DNA, transformed host microorganism and method for producing a polypeptide having mutarotase activity

US5508176A · kind A · utility

Assignee

• Merck Patent GmbH · DE

Inventors

• Wolfgang Hillen · Erlangen, DE
• Robert Schmucker · Hamburg, DE
• Ulrike Guelland · Erlangen, DE

Key dates

• Filing date: Sep 12, 1988
• Grant date: Apr 16, 1996
• Expiry date: Sep 12, 2008

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/849
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A polypeptide having mutarotase activity is obtained from a host microorganism that has been transformed with a molecule having a recombinant DNA sequence. The molecule having a recombinant DNA sequence is prepared by removing the first 60 nucleotides of a DNA sequence originating from the genome of Acinetobacter calcoaceticus that codes for the polypeptide, modifying the following 21 nucleotides and fusing of the resultant structural gene with the start of the tetracycline-repressor gene and with an effective promotor sequence. The tetracycline-regressor gene and the promotor sequence preferably originate from the same microorganism such as E. coli in which expression of the polypeptide is carried out, and result in increased yield of the expressed polypeptide having mutarotase activity.