In vitro phage lambda DNA packaging system

US5508187A · kind A · utility

Assignee

• Pharmacia P.L. Biochemicals, Inc. · US

Inventors

• Susan Frackman · Madison, US
• Phillip P. Franciskovich · Greenville, US
• James F. Jolly · Glendale, US
• Robert Luhm · Mequon, US
• William A. Riedl · Cedarburg, US

Key dates

• Filing date: Dec 23, 1993
• Grant date: Apr 16, 1996
• Expiry date: Dec 23, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/22
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A bacterial preparation capable of packaging phage .lambda. DNA is disclosed. This preparation is in lyophilized form and is stable at ambient temperature. In a preferred form, the preparation contains an over-expressed terminase protein, is prepared from the bacterial strain E. coli Cla [.lambda. c/857 Sam7 .DELTA.(cos-Nu1-A)::Kn.sup.r ]/.lambda. pTER and is capable of a packaging efficiency of at least 1.times.10.sup.8 pfu/.mu.g wild type .lambda. DNA. The present invention is also a method of creating a phage .lambda. DNA packaging extract comprising the steps of preparing a bacterial extract capable of packaging phage .lambda. and lyophilizing said extract.