Process for immobilizing a nucleic acid fragment by passive attachment to a solid substrate, the solid substrate thus obtained, and its use
US5510084A · kind A · utility
36Cited by
9References
20Claims
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Key dates
| Filing date | Jul 12, 1994 |
| Grant date | Apr 23, 1996 |
| Priority date | — |
| Expiry date | Jul 12, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In a process for immobilization by passive fixation on a solid support of a nucleic acid fragment containing less than 100 nucleotides, the fragment is deposited on the support in the form of a derivative. The derivative results from the covalent coupling of the fragment with a ligand having a molecular mass of less than 5,000 and containing at least one polar functional group. The derivative is not capable of forming a covalent bond with the support under the conditions of the deposition. When the ligand is a nucleotide or an oligonucleotide it is at least one nucleotide modified so as to introduce the polar functional group.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.