Liquid column packing materials
US5512169A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Dec 30, 1992 |
| Grant date | Apr 30, 1996 |
| Priority date | — |
| Expiry date | Dec 30, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/812
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Packing materials for liquid chromatographic or catalytic columns are prepared by contacting a porous protein-adsorptive particulate or membranous support, such as a porous silica particulate support, with an aqueous solution into which a protein has been dissolved to form a saturated coating of protein on the external surfaces of the porous protein-adsorptive support, removing excess protein that remains in solution by washing, and, then crosslinking the protein in the coating. The result is a packing material which resists further adsorption by many different proteins but which continues to provide the adsorptive or catalytic properties of the groups on the internal surfaces of the porous protein-adsorptive support for separations, analysis, or alteration of small molecules. The packing material of the present invention is particularly useful in HPLC or solid phase extraction columns for direct injection drug analysis in plasma, serum, and urine.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.