Assay for enzyme activity
US5512429A · kind A · utility
8Cited by
11References
24Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jun 20, 1994 |
| Grant date | Apr 30, 1996 |
| Priority date | — |
| Expiry date | Jun 20, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of assay for an enzyme catalysing the release of long chain fatty acids or, conversely, for an ester substrate for such enzymes is applicable to clinical samples and comprises removing substantially all the albumin from the clinical sample, incubating the sample after albumin removal with, for enzyme assay, an ester substrate or, for substrate assay, the enzyme, under conditions effective to release a fatty acid, causing the fatty acid to bind to a fatty acid binding protein and assaying the binding of the fatty acid to the binding protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.